Pin1 Prolyl Isomerase Regulates Endothelial Nitric Oxide Synthase

Pin1 prolyl isomerase regulates endothelial nitric oxide synthase.

OBJECTIVE The Pin1 prolyl isomerase acts in concert with proline-directed protein kinases to regulate function of protein substrates through isomerization of peptide bonds that link phosphoserine or phosphothreonine to proline. We sought to determine whether Pin1 interacts with endothelial nitric oxide synthase (eNOS) in endothelial cells in a manner that depends on proline-directed phosphoryla...

The Peptidyl-Prolyl Isomerase Pin1 Regulates

The peptidyl-prolyl isomerase Pin1 regulates cytokinesis through Cep55.

Failure of cytokinesis results in tetraploidy and can increase the genomic instability frequently observed in cancer. The peptidyl-prolyl isomerase Pin1, which is deregulated in many tumors, regulates several processes, including cell cycle progression. Here, we show a novel role for Pin1 in cytokinesis. Pin1 knockout mouse embryonic fibroblasts show a cytokinesis delay, and depletion of Pin1 f...

Interaction of Endothelial Nitric Oxide Synthase with Mitochondria Regulates Oxidative

39 An increase in oxygen tension at birth is one of the key signals that initiate pulmonary 40 vasodilation in the fetal lung. We investigated the hypothesis that targeting endothelial 41 nitric oxide synthase (eNOS) to the mitochondrial outer membrane regulates reactive 42 oxygen species (ROS) formation in the fetal pulmonary artery endothelial cells (PAEC) 43 during this transition. We isolat...

Endothelial nitric oxide synthase regulates microlymphatic flow via collecting lymphatics.

Functional interactions between the initial and collecting lymphatics, as well as the molecular players involved, remain elusive. In this study, we assessed the influence of nitric oxide (NO) on lymphatic fluid velocity and flow, using a mouse tail model that permits intravital microscopy and microlymphangiography. We found that NO synthase (NOS) inhibition decreased lymphatic fluid velocity in...